Our ResearchReady Plasmid DNA Service utilizes our proprietary resin, PolyFlo® (DMF13090) and results in highly pure DNA meeting the specifications detailed below. ResearchReady Plasmid DNA is suitable for a variety of research applications.
There are three levels of guaranteed specifications available each with customizable options that can be included as needed: Basic, Intermediate, and Premium.
BASIC ResearchReady Specifications– This quality of DNA is suitable for many standard research applications such as transfections, transformation, and diagnostics at the bench level.
| PARAMETER | SPECIFICATION |
| Appearance | Clear, Colorless, No Visible Particulates |
| A260:280 | 1.7-2.0 |
| Concentration | Amount Ordered (mg) / 10ml |
| DNA Homogeneity | ≥80% |
| Residual RNA | None Detected @ 0.2ug load |
| PARAMETER | SPECIFICATION |
| Identity | Consistent with Provided Information |
| High Concentration | Collaborator Defined |
| Bottling | Collaborator Defined |
| Bioburden Testing | Overnight or Five Day Incubation |
Intermediate
INTERMEDIATE ResearchReady Specifications are more stringent and include additional Quality Analysis. This quality of DNA is suitable for transfections, transformation, and diagnostics for process development. A minimum of 20mg is required for each order.
Included
| PARAMETER | SPECIFICATION |
| Appearance | Clear, Colorless, No Visible Particulates |
| A260:280 | 1.7-2.0 |
| Concentration | Amount Ordered (mg) / 10ml |
| DNA Homogeneity | ≥85% |
| Residual RNA | None Detected at 0.2µg load |
| ssDNA, Linear DNA, Chr DNA | None Detected at 0.2µg load |
| Endotoxin | <100EU/mg |
| PARAMETER | SPECIFICATION |
| Identity | Consistent with Provided Information for 2 Rxns |
| High Concentration | Collaborator Defined |
| Aliquoting | Collaborator Defined |
| Bioburden Testing | Overnight or Five Day Incubation |
PREMIUM ResearchReady Specifications are the most stringent and include the maximum Quality Analysis. This quality of DNA is suitable for transformations, transfections, and diagnostics for toxicology studies and animal studies. A minimum of 20mg is required for each order.
Included
| PARAMETER | SPECIFICATION |
| Appearance | Clear, Colorless, No Visible Particulates |
| A260:280 | 1.7-2.0 |
| Concentration | Amount Ordered (mg) / 10ml |
| DNA Homogeneity | ≥90% |
| Residual RNA | None Detected @ 1.0ug load |
| ssDNA, Linear DNA, Chromosomal DNA | None Detected @ 1.0ug load |
| Endotoxin | <30EU/mg |
| Identity | Consistent with Provided Information for 2 reactions |
| PARAMETER | SPECIFICATION |
| High Concentration | Collaborator Defined |
| Bottling | Collaborator Defined |
| Bioburden Testing | Overnight or Five Day Incubation |
| More Stringent Endotoxin | <5EU/mg |
| Sterile Fill | Sterile Filled by Quality |