Q: What is the optimal harvest time for maximum Adenovirus yield?

In general, a much higher virus yield is obtained using a 4-5 day procedure, and for this reason alone we recommend it. In general, a 48 hour procedure results in a higher level of contaminating cellular and viral DNA relative to the 4-5 day procedure.

Q: How much Adenovirus will the Adenopure LS^® kit purify?

The Adenopure LS purification procedure will yield up to 1x10¹⁴ viral particles. Of course this is dependant upon loading at least that much virus onto the Virus-Binding Module of the Adenopure LS kit. Each kit contains one (1) purification procedure.

Q: Can I pass more than (50) 150cm² plates worth of infected cell extract through the virus binding module?

The Adenopure LS purification procedure has been optimized to process (50) 150cm² plate equivalents of infected cell extract. If more plate equivalents of infected cell extract are used in a purification procedure, the contaminating free cellular and viral DNA may not be totally degraded and the amount and purity the resulting Adenovirus preparation may be compromised. In addition, the user may run short on some of the buffers supplied in the kit.

Q: How critical is it to add the right amount of 10X Dilution Buffer?

It is extremely critical to add the right amount of 10X Dilution Buffer to the sample as this is key to getting efficient binding of Adenovirus to the Virus-Binding Module. The addition of an incorrect amount of 10X Dilution Buffer will lead to a decrease in the strength of interaction of Adenovirus with the Virus-Binding Module as well as allowing a different spectrum of contaminating proteins to compete with the Adenovirus for binding to the Virus-Binding Module.

Q: Can I reuse the Adenopure LS Purification Unit?

The Adenopure LS Purification Unit is intended for single use only and should be disposed of after a purification procedure in an appropriate biohazardous waste receptacle.

Q: What happens if I push the sample through the Adenopure LS virus binding module at a higher flow rate than 50ml/minute?

The efficiency of binding of Adenovirus to the Virus-Binding Module will decrease with increasing flow rate, and the overall recovery of purified Adenovirus will descrease as a result.

Q: What is the composition of the buffers in the kit?

The formulation of the Adenopure LS Kit buffers contained within the Adenopure LS kit is proprietary. MSDS are available for review.